Have you ever waited so long for a bathroom break that you thought your bladder would burst? Ignoring your body's warnings and holding your urine for an extended period might harm your bladder and overall health. Before reaching capacity, a healthy human bladder can retain between 400 and 500 milliliters of pee or roughly 2 cups. Even though a healthy bladder can stretch and accommodate higher volumes of urine, it's critical to urinate regularly.